Prem P. Singh

Postdoctoral Scholar at UC Davis

Prem Pratap Singh

Plant scientist with expertise in plant-pathogen interactions and multi-omics analysis. Currently investigating grapevine-virus interactions using transcriptomic and metabolomic approaches. Proficient in R, Python, GC-MS, LC-MS/MS, RNA-seq, and bioinformatics pipelines.

Prem Pratap Singh

About Me

I am a Postdoctoral Scholar at the University of California, Davis, investigating grapevine-virus interactions using transcriptomic and metabolomic approaches, building on foundational GRBV research. I lead CDFA/USDA-funded projects on grapevine virus impacts.

My doctoral research at Banaras Hindu University focused on developing nano-encapsulated antifungal formulations against food-borne pathogens. I engineered chitosan-based delivery systems that significantly inhibited Aspergillus flavus growth and aflatoxin contamination.

With a track record of 20+ publications, grant contributions, and mentorship experience, I am passionate about translating fundamental research into practical solutions for sustainable agriculture and food safety.

Research Interests

Grapevine-Virus InteractionsMulti-Omics IntegrationPostharvest Biology & Food SafetyNanoencapsulationPlant VolatilesComputational Biology

Skills & Expertise

Analytical Techniques

GC-MSLC-MS/MSRT-qPCRDigital PCRRNA-seqSEM ImagingSpectral Analysis

Data Science & Bioinformatics

R (Bioconductor, DESeq2, ggplot2)PythonMulti-omics IntegrationStatistical ModelingPathway Enrichment Analysis

Research Areas

Plant PathologyGrapevine VirologyPostharvest BiologyFood SafetyAflatoxin Analysis

Formulation & Delivery

Nano-encapsulationChitosan MatricesEssential Oil FormulationsSustained-release Systems

Experience

Sep 2023 — Present

Postdoctoral Scholar

University of California, Davis

Davis, CA, USA

Leading multi-omics research on grapevine-virus interactions under CDFA and USDA-funded projects.

  • Led a multi-year, omics-driven project to dissect metabolic and transcriptional impacts of Grapevine Red Blotch Virus (GRBV) on Vitis vinifera cv. Merlot berries and wine quality
  • Developed and validated RT-qPCR and digital PCR assays to quantify GRBV titers across >10 vineyard blocks
  • Integrated multi-platform datasets (GC-MS, LC-MS/MS, RNA-Seq) to identify putative biomarkers linked to virus-induced changes
  • Presented results at national conferences (ASEV and ASPB), contributing to stakeholder outreach and industry workshops

2017 — 2023

Ph.D. Research Scholar

Banaras Hindu University

India

Doctoral research on nano-encapsulated plant-based bioactive formulations against food-borne pathogens.

  • Engineered a nano-encapsulated essential oil formulation using chitosan-gel matrices, significantly inhibiting Aspergillus flavus growth and aflatoxin B1 production
  • Set up a multi-disciplinary R&D pipeline integrating antifungal screening, GC-MS metabolomics, SEM imaging, and gene expression analysis
  • Developed a green, cost-effective, and scalable chitosan-based delivery system with sustained release over 60 days
  • Published 10+ peer-reviewed articles and presented findings at 5+ national conferences

Education

2017 — 2023

Ph.D. in Plant Pathology/Postharvest/Food Safety

Banaras Hindu University

India

Thesis: Assessment and amelioration of plant-based bioactive formulation against food-borne pathogens

2015 — 2017

M.Sc. in Botany (Gold Medalist)

Banaras Hindu University

India

Specialization: Plant Pathology and Plant Protection, Microbial Genetics and Biotechnology, Conservation and Restoration Ecology

2012 — 2015

B.Sc. in Botany

Banaras Hindu University

India

Foundation in plant sciences and biological research methods.

Research Impact

2,326

Total Citations

25

h-index

40

i10-index

Since 2021

1,895

Citations

24

h-index

38

i10-index

Citations per Year

012525037550020192020202120222023202420252026

Publications

Type:
Method:

Grapevine Red Blotch Disease: An Emerging Threat to Global Viticulture

P. P. Singh, K. Reddy, A. M. Boghozian, A. Oberholster, M. R. Sudarshana

In Pipeline(2025)
RNA-SeqMulti-omicsReview

Temporal Dynamics of Grapevine red blotch virus accumulation in Grapevine leaves is Influenced by Fruit Maturity Stages

P. P. Singh, H. Scully, K. Reddy, A. M. Boghozian, C. Medina-Plaza, A. Oberholster, M. R. Sudarshana

In Pipeline(2025)
RT-qPCRDigital PCRStatistical Modeling

Systematic elucidation of aflatoxigenic Aspergillus flavus inhibition: Integrating EOs-based formulation with mathematical modeling and transcriptomic analysis

P. P. Singh, R. Singh, P. K. Verma, B. Prakash

In Pipeline(2025)
TranscriptomicsMathematical ModelingGC-MS

Assessment of Trachyspermum ammi essential oil against Aspergillus flavus, aflatoxin B1 contamination, and post-harvest quality of Sorghum bicolor

P. P. Singh, A. K. Jaiswal, R. Singh, A. Kumar, V. Gupta, T. S. Raghuvanshi, A. Sharma, B. Prakash

Food Chemistry(2024)Paper
GC-MSAntifungal ScreeningPostharvest Analysis

Essential oils as green promising alternatives to chemical preservatives for agri-food products: New insight into molecular mechanism, toxicity assessment, and safety profile

B. Prakash, P. P. Singh, V. Gupta, T. S. Raghuvanshi

Food and Chemical Toxicology(2024)Paper
Molecular DockingToxicity AssessmentReview

Insights into the antimicrobial efficacy of Coleus aromaticus essential oil against food-borne microbes: Biochemical and molecular simulation approaches

P. P. Singh, A. K. Jaiswal, T. S. Raghuvanshi, B. Prakash

Food and Chemical Toxicology(2023)Paper
Molecular SimulationGC-MSAntimicrobial Assay

32 publications (24 journal articles, 8 book chapters) in venues including Food Chemistry, Scientific Reports, and Food Control.

Research Projects

GRBV Impact on Wine Quality

Multi-year, omics-driven project funded by CDFA and USDA to dissect the metabolic and transcriptional impacts of Grapevine Red Blotch Virus on Vitis vinifera cv. Merlot berries and wine quality.

Identified key metabolic pathways disrupted by GRBV, informing vineyard management for the $58B California wine industry.

RNA-SeqGC-MSLC-MS/MSRPython

GRBV Quantification Assays

Developed and validated RT-qPCR and digital PCR assays to quantify GRBV titers across >10 vineyard blocks, enabling spatial and seasonal tracking of infection load.

Enabled early detection and spatial mapping of virus spread, helping growers make informed replanting decisions.

RT-qPCRDigital PCRStatistical Modeling

Nano-encapsulated Antifungal Formulation

Engineered a chitosan-gel matrix nano-encapsulated essential oil formulation that significantly inhibited Aspergillus flavus growth and aflatoxin B1 production in postharvest applications.

Achieved >90% aflatoxin inhibition using green, food-safe materials — a viable alternative to synthetic preservatives.

Nano-encapsulationChitosanGC-MSSEM

Multi-omics Biomarker Discovery

Integrated multi-platform datasets (GC-MS, LC-MS/MS, RNA-Seq) to identify putative biomarkers linked to virus-induced changes in grape cell wall composition and metabolite profiles.

Discovered novel biomarkers that could serve as diagnostic tools for early GRBV detection in field conditions.

BioconductorDESeq2Pathway AnalysisData Visualization

Sustained-Release Delivery System

Developed a green, cost-effective, and scalable chitosan-based delivery system with enhanced formulation stability and sustained release over 60 days in postharvest conditions.

Extended antifungal protection from days to 60+ days, reducing reapplication frequency and cost for food storage.

Chitosan MatricesEssential OilsFormulation Chemistry

Antifungal Mechanism Elucidation

Multi-disciplinary R&D pipeline integrating antifungal screening, GC-MS metabolomics, SEM imaging, and gene expression analysis to uncover mechanisms involving oxidative stress and membrane disruption.

Revealed a dual-target mechanism (membrane disruption + oxidative stress) opening new avenues for rational antifungal design.

TranscriptomicsMetabolomicsGene ExpressionMolecular Docking

Get In Touch

I am open to research collaborations, academic discussions, and consulting opportunities. Whether you have questions about my research or want to explore potential collaborations, feel free to reach out!

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